Gene Information
Gene Name | GFP |
Species | Aequorea victoria |
Gene Length | 717 bp |
Product Feature | Full length Clone DNA of green fluorescent protein with C terminal GFPSpark tag. |
Plasmid Information
Vector | pCMV3-C-GFPSpark® |
Promoter | Enhanced CMV promoter |
Tag Sequence | GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG |
Sequencing Primer | T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' ) |
Quality Control | The plasmid is confirmed by full-length sequencing. |
Screening
Bacterial Screening Resistance | Kanamycin |
Cell Screening Resistance | Hygromycin |
Application | Stable or Transient mammalian expression |
Background
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
![GFP cDNA ORF Clone, Aequorea victoria, C-GFPSpark® tag [BY-O-10001]](http://30712400.s21i.faiusr.com/4/1/ABUIABAEGAAg4pGqogYosM6D-AcwmwQ46AU!400x400.png)
