Exosomes are extracellular membrane vesicles (EVs) with a diameter of 30-150 nm secreted by various cells. Exosomes are commonly found in many body fluids, and their contents are rich in proteins, lipids, nucleic acids, and other components. They play an important role in intercellular communication and are primarily involved in immune antigen presentation, neurotransmitter transmission, lipid metabolism, cell signal transduction, and other processes. Exosomes are closely related to the occurrence, development, treatment, and prognosis of various diseases.
Product Introduction
Density ultracentrifugation is a traditional and effective method for exosome isolation, but its complex operation process and expensive equipment limit the routine study of exosomes. The serum/plasma rapid extraction kit independently developed by DoLeMi can quickly and efficiently obtain high-purity exosome particles through component optimization. It is easy to operate, takes a short time, produces stable results, and has low levels of contaminating proteins. With only an ordinary centrifuge, relatively pure and intact exosomes can be isolated.
Product Application
The extracellular vesicles extracted by this reagent kit can be used for NTA particle size analysis, Western Blot, nucleic acid extraction and subsequent analysis (such as Q-PCR, sequencing), extracellular vesicle labeling, and protein mass spectrometry analysis.