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EndoFree Plasmid Mini Purification Kit [BY-B-10054]

Cat.No.：	BY-B-10054
Size：	50 preps

Product Detail

Documents

Kit Components

Item	Specifications (50 preps)
Buffer BL	30 mL
Buffer P1	30 mL
Buffer P2	30 mL
Buffer P4	30 mL
Buffer PD	30 mL
Buffer PW	15 mL
Buffer TB	15 mL
RNase A(10 mg/mL)	300 μL
Filtration Columns CS	50
Spin Columns CP4	50
Collection Tubes (2mL)	100
Hand book	1

Extract Yield

Plasmid Type	Bacterial Volume	Yield
low copy	5-15 mL	5-25 μg
High Copy	5-15 mL	15-70 μg

Introduction

Ø This kit uses a unique silica gel membrane adsorption technology to efficiently and specifically bind plasmid DNA. At the same time, special Buffer P4 and Filtration Columns CS are used to effectively remove impurities such as endotoxin and protein. The whole extraction process only takes 1 hour, which is convenient and quick. The following procedure is suitable for extracting 5-15 mL of overnight cultured E. coli. The yield and quality of plasmid extraction are related to the type of host bacteria and culture conditions, cell lysis, plasmid copy number, plasmid stability, antibiotics and other factors.

Ø The plasmid DNA extracted using this kit can be applied to transfect various cells and various routine operations, including enzyme digestion, PCR, sequencing, ligation and other experiments.

Product Features

Ø Fast: The whole operation process is fast and convenient, and the recovery work can be completed in tens of minutes.

Ø Efficient: The unique spin column and carefully prepared buffer can recover a large amount of high-purity target plasmid DNA.